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Cell-Free Protein Production

Methods and Protocols

Yaeta Endo; Kazuyuki Takai; Takuya Ueda

978-1-60327-331-2

2010
Edition 1
- Covers cell-free expression systems from E. coli, wheat, insect, and human cells as well as a totally reconstituted system
- Shows how cell-free expression can be applied to high-throughput and one-by-one protein functional and structural analyses
- Provides rapid methods for protein engineering and the creation of new proteins
- Presents basic methodologies that can support constitutive approaches for understanding complex molecular systems
- Includes supplementary material: sn.pub/extras
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