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  • eBook:Bioluminescence

    Bioluminescence

    Methods and Protocols
    Preston B. Rich; Christelle Douillet
    978-1-60327-321-3
    2009
    Edition 2
    • Provides an easily accessible reference volume for a wide range of bioluminescence assays and applications written by leading researchers in the field
    • Comprehensive guide to developing, validating, running, and analyzing bioluminescence assays
    • Develops detection and imaging methods
    • Describes the use of a wide variety of bioluminescence tools: various luciferases, reporter gene systems including PIN-G reporters, light emitting bacteria, smart probes, BRET, quantums dots, transgenic animals, etc.
    • Presents protocols with enough details to be followed and adapted by scientific teams with no previous experience in bioluminescence
    • Contains numerous notes about potential pitfalls and tips on how to avoid failures
    • Presents cutting-edge material that will also interest experts in the field of bioluminescence
    • Provides methods and assays that can be adapted and used in most specialties of biomedical and biotechnological fields: studies of cell trafficking, protein-protein interactions, intracellular signaling, apoptosis, RNAi knockdown, bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors
    • Covers a wide range of sub-cellular and cell-based assays for in vitro and in vivo analysis as well as non invasive, in vivo, and real time assays and monitoring for animal models
    • Also includes methods for high throughput analysis of animal models
    • Includes supplementary material: sn.pub/extras

    Get access as part of Springer Protocols.

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