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Expressed Sequence Tags (ESTs)

Generation and Analysis

John Parkinson

978-1-60327-136-3

2009
Edition 1
- Essential reading for any scientist beginning their own EST project
- Takes the reader through the entire process of how to successfully complete an EST project
- Covers a wide range of cDNA library construction techniques from the generation of full length libraries to the use of splice leaders and normalization and subtraction procedures
- Details state-of-the-art bioinformatics pipelines and tools that can be readily implemented for high throughput processing and analysis of ESTs
- Shows the reader how EST technology can be exploited for expression studies, particularly from the perspective of human health
- Provides strategies, tips and potential pitfalls in the generation and analysis of ESTs from leading exponents of the field
- Includes supplementary material: sn.pub/extras
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