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Plant MicroRNAs

Methods and Protocols

Blake C. Meyers; Pamela J. Green

978-1-60327-005-2

2010
Edition 1
- Presents fundamentals of plant miRNA biology, including pathways and approaches used for their discovery and investigation, as written by leading researchers in the field
- Covers a broad range of molecular approaches to the study of small RNA, from basic to advanced experimental and computational techniques
- Details the methods by which the expression of miRNAs and their impact on mRNA levels is characterized, including deep sequencing methods for miRNAs and target RNAs
- Enables a molecular biologist to design and perform experiments on the biology of plant miRNAs and small RNAs, including detection, quantification and localization, as well as approaches to data analysis
- Includes applications and advanced adaptations of techniques relevant to all plant biologists
- Includes supplementary material: sn.pub/extras
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