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  • eBook:Potassium Channels

    Potassium Channels

    Methods and Protocols
    Jonathan D. Lippiat
    978-1-59745-526-8
    2009
    Edition 1
    • Contains detailed protocols that describe the use of routine and advanced electrophysiological techniques, such as whole cell patch clamp, perforated patch clamp, excised macropatch, open-cell cell-attached patch clamp, planar patch clamp, and artificial bilayer
    • Of major pharmacological interest as a number of chapters describe the use of potassium channel drug screening platforms, planar electrophysiology, and the cardiac safety pharmacology of HERG potassium channels
    • Describes optical techniques that allow the real-time study of protein interactions and conformational changes in potassium channels
    • Examines both functional experiments and protocols that allow the evaluation of the cellular biology of potassium channels, like transcription, splicing, and protein trafficking
    • Features standard laboratory techniques such as cell culture and transfection, oocyte injection, Western blotting, PCR to support these experiments
    • Includes supplementary material: sn.pub/extras

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