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High Throughput Protein Expression and Purification

Methods and Protocols

Sharon A. Doyle

978-1-59745-196-3

2009
Edition 1
- Provides step-by-step protocols for protein production that utilize a wide range of cutting-edge techniques
- Covers cell-based and cell-free methodologies needed to produce bacterial proteins, eukaryotic proteins, soluble and membrane proteins
- Examines a variety of cloning methodologies that enable high-throughput cloning for protein production
- Describes the use of a variety of tags to facilitate parallel processing for high-throughput protein production
- Includes several reviews to help readers choose which protocols best suits their needs
- Provides protocols that utilize robotics for sample handling, as well as protocols designed small laboratories performing experiments by hand
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